26.3 Folate

26.3.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 129.2308225 1 <0.0001 ***
Folate 2.0006170 1 0.2 ns
Study 0.5519894 1 0.5 ns
GA_samp 130.9134873 1 <0.0001 ***
Folate:Study 2.1143312 1 0.1 ns
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 32.2090781 1 <0.0001 ***
Folate 0.1917908 1 0.7 ns
Study 8.1357120 1 0.004 **
GA_samp 1633.8508884 1 <0.0001 ***
Folate:Study 0.0205584 1 0.9 ns
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 329.3390138 1 <0.0001 ***
Folate 0.4074204 1 0.5 ns
Study 1.3523959 1 0.2 ns
GA_samp 806.7098807 1 <0.0001 ***
Folate:Study 1.0842100 1 0.3 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 Folate 1 Inf 8.298 8.298 0.004 **
3 Study 1 Inf 11.103 11.103 0.0009 ***
4 GA_samp 1 Inf 0.126 0.126 0.7 ns
2 Folate:Study 1 Inf 1.122 1.122 0.3 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 Folate 1 Inf 8.507 8.507 0.004 **
3 Study 1 Inf 19.086 19.086 <0.0001 ***
4 GA_samp 1 Inf 0.220 0.220 0.6 ns
2 Folate:Study 1 Inf 1.183 1.183 0.3 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 Folate 1 Inf 0.001 0.001 1.0 ns
3 Study 1 Inf 42.997 42.997 <0.0001 ***
4 GA_samp 1 Inf 0.215 0.215 0.6 ns
2 Folate:Study 1 Inf 1.230 1.230 0.3 ns

26.3.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 12.75 (8.19, 19.85) <0.0001
Folate 1.00 (1.00, 1.00) 0.69
StudySTOP 1.02 (0.89, 1.16) 0.83
GA_samp 1.17 (1.14, 1.20) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 1.94 (1.54, 2.43) <0.0001
Folate 1.00 (1.00, 1.00) 0.59
StudySTOP 1.14 (1.07, 1.23) 0.0002
GA_samp 1.34 (1.32, 1.36) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.06 (0.05, 0.08) <0.0001
Folate 1.00 (1.00, 1.00) 0.72
StudySTOP 1.03 (0.94, 1.13) 0.52
GA_samp 1.32 (1.29, 1.34) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 17.06 (9.66, 30.13) <0.0001
Folate 1.00 (1.00, 1.00) 0.006
StudySTOP 0.72 (0.60, 0.86) 0.0004
GA_samp 0.99 (0.96, 1.03) 0.72
HOMA-IR
Var Effect P
(Intercept) 4.28 (2.37, 7.76) <0.0001
Folate 1.00 (1.00, 1.00) 0.006
StudySTOP 0.62 (0.51, 0.75) <0.0001
GA_samp 0.99 (0.95, 1.03) 0.64
Glucose (mmol/L)
Var Effect P
(Intercept) 5.56 (4.99, 6.19) <0.0001
Folate 1.00 (1.00, 1.00) 0.96
StudySTOP 0.87 (0.84, 0.90) <0.0001
GA_samp 1.00 (0.99, 1.00) 0.60

26.3.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Serum folate concentration (nM) and may be misleading when interaction term is involved.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 95.57106 7.958719 104.79538 81.02409 112.7298
STOP 97.01584 7.541447 80.00772 83.11113 113.2469
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 89.49349 2.679891 194.3292 84.36113 94.93809
STOP 102.42581 2.702637 117.4517 97.21102 107.92033
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 2.267774 0.0772606 440.4969 2.120901 2.424818
STOP 2.338526 0.0643717 218.5331 2.215038 2.468900
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 13.95201 0.8885756 Inf 12.314747 15.80695
STOP 10.04604 0.4491605 Inf 9.203169 10.96610
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 3.363128 0.2265870 Inf 2.947098 3.837886
STOP 2.094340 0.0981371 Inf 1.910563 2.295795
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.424406 0.0686112 Inf 5.291584 5.560562
STOP 4.716491 0.0413280 Inf 4.636181 4.798192

26.3.4 Comparisons

Effect for every 50 unit increase in Serum folate concentration (nM).
Hormone Contrast Study Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) Folate89.2 / Folate39.2 SCOPE 0.99 (0.94, 1.05) 0.7
PRL (ng/ml) Folate89.2 / Folate39.2 STOP 0.99 (0.94, 1.05) 0.7
hPL (μg/ml) Folate89.2 / Folate39.2 SCOPE 0.99 (0.96, 1.02) 0.6
hPL (μg/ml) Folate89.2 / Folate39.2 STOP 0.99 (0.96, 1.02) 0.6
GH2 (ng/ml) Folate89.2 / Folate39.2 SCOPE 0.99 (0.95, 1.03) 0.7
GH2 (ng/ml) Folate89.2 / Folate39.2 STOP 0.99 (0.95, 1.03) 0.7
Insulin (uU/ml) Folate89.2 / Folate39.2 SCOPE 0.91 (0.86, 0.97) 0.006
Insulin (uU/ml) Folate89.2 / Folate39.2 STOP 0.91 (0.86, 0.97) 0.006
HOMA-IR Folate89.2 / Folate39.2 SCOPE 0.91 (0.85, 0.97) 0.006
HOMA-IR Folate89.2 / Folate39.2 STOP 0.91 (0.85, 0.97) 0.006
Glucose (mmol/L) Folate89.2 / Folate39.2 SCOPE 1.00 (0.99, 1.02) 1.0
Glucose (mmol/L) Folate89.2 / Folate39.2 STOP 1.00 (0.99, 1.02) 1.0

26.3.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

26.3.6 Plots